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KMID : 0382619820020010011
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Hanyang Journal of Medicine
1982 Volume.2 No. 1 p.11 ~ p.25
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Transmission of Multiple Drug-resistance in Shigella Strains in Korea
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金仁世/Kim, In Sae
趙陽子/徐仁銖/Cho, Yang Ja/Suh, Inn Soo
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Abstract
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A total of one hundred and eighty-three strains of Shigella isolated from patients in Seoul area between 1971 and 1979 were studied for drug-resistance and distribution of R plasmids. On the other hand this report dealt with the experimental results of the transmission of R plasmids from multiple-resistant strains of Shigella to Escherichia coli K-12 and the retransmission of the R plasmids from resistantized .Escherichia cole K-12 to sensitive Salmonella typhi-muriunz and Shigella flexneri.
Antibiotic susceptibilities were determined by an agar dilution method. Mueller Hinton agar were used for the assay of drug-resistance and propagating medium for conjugation. Shigella isolates found to be one or more antibiotics; i. e. to -tetracycline (TC), chloramphenicol (CP), streptomycin (SM), or sulfonamide (SA) were considered potential donors of R plasmids to Escherichia coli K-12 RFP=. Escherichia coli K-12 RFP= obtained through the courtesy of Prof. Nagaya, Tokyo Medical and Dental Medical School, Japan was used as the resipients of R plasmids. The transfer of R plasmids was carried out by the modified technique of Watanabe. The method of retransfer of the R plasmids was same as the transmission of the R plasmids from Shigella to Escherichia coli In retransmission experiment, Escherichia coli K-12 receiving R plasmids from multiple resistant :strains of Shigella were used as donors. Sensitive Salmonella typhi-murium No. 156 and Shigella flexneri No. 56 which were resistant to 400pg and 200,ag per ml ,of nalidixic acid respectively were employed as the recipients of R plasmids.
1. Of 115 drug-resistant strains of Shigella, 70.4% harbored R plasmids. R _plasmids detected were classified in to nine groups; R (TC, CP, SM, SA) 48.2f/o, R (SM, SA) 18.5%, R (SMSA) SA) 18.5%, R (TC, CP, SA) 17.35y,, R (CP, SM, SA) 3.7-/,,, =R (SM) 3.7%, R (TC, SM, SA), R (TC, SA) and R (SA) 2..4% respectively.
2. TC-, CP-, and SA-resistances of Escherichia coli K-12 wich received the R plasmids from Shigella were regulary lower than those of donors but transmitted :resistance of ampicillin (AP) exhibited the same level of resistance as donors.
3. According to the colonies, the level of the transmitted multiple-resistance of Escherichia coli received the plasmids varied in consideracly wide range and only 6.7% of the total colonies were shown the level of multiple resistance as high as -those of donor.
4. The transter of resistance from Shigella flexneri to E. coli K-12 were from ´-10-2.5 to 10-3?c or from 10-2.3 to 10-4.1 when selecting with TC . or TC and SM respectively.
5. The retransmission was accomplished from Escherichia coli K-12 which received -the R plasmids from Shigella to Salmonella typhi-murium NAr No. 156 and .Shigella flexneri NAz No. 56. In retransmission, former recipients exhibited multiple resistance nearly as high as those of the original donors, however, the later one exhibited multiple resistances regulary lower than those of the original donors and maintained by repeated subcultures on drugfree nutrient broth.
6. The transmission of resistance to AP and cephaloridine (CER) were noted in ´21 strains (58.3/) out of 58 strains. However nalidixic acid (NA) resiistance was not transferred.
7. The frequency of drug-resistance of 183 Shigella strains to 12 drugs were shown high incidence of 65x57/ to SM, SA, TC, or CP, moderate incidence of 3615/ to nitrofurantoin(NF), AP, or CER and low incidence of 2x0.5% to NA, kanamycin (KM), gentamicin (GM) or f uraziomycin (FM). However resistance to furazolidone (FZ) was not observed.
Isolation of resistant strains of Shigella by year were as follows; AP resistance was first appeared in 1978 in7high frequency and persisted till 1979. FM resistance -was first observed in 1978 and resistance to kanamycin(KM), GM, and NA were first detected in .1979, respectively but TC, Cp, SM, SA, CER and NF were isolated from 1971 and continuously increased till 1979.
Among 139 strains examined, highest frequency of multiple resistance was of ,quadruply (64/).
8. Drugs which revealed more less 10 ,ug/ml in median in hibitory concentrations .by Spearman-Karber´s method. were GM, NA, KM, FZ and CER.
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